定制您的化合物库 通过在我们的库存中挑选化合物来建立合适的化合物库,用于您的研究工作。 您可以选择: 特定化合物 96/384孔板位图 可选择\"干/固体”或\"DMSO溶液” 请通过info@selleck.cn与我们联系,定制你所需要的化合物库。 A-769662是一种有效的,可逆的AMPK激活剂,在无细胞试验中EC50为0.8 μM,对GPPase/FBPase活性几乎没有作用。 今日订购,明日送达,全国免运费!全国免费电话:400-668-6834|Email:info@selleck.cn 产品描述 A-769662是一种有效的,可逆的AMPK激活剂,在无细胞试验中EC50为0.8 μM,对GPPase/FBPase活性几乎没有作用。 A-769662部分刺激纯化的大鼠肝脏AMPK,EC50 为0.8 μM。A-769662以剂量响应的方式激活从多种组织和物种纯化得到的AMPK,伴随不同的EC50s。A-769662的EC50s,使用从大鼠心脏,大鼠肌肉,或人胚肾细胞(HEKs)部分纯化的AMPK测定,结果分别为2.2 mM,1.9 mM,或1.1 mM。原代大鼠肝细胞用A-769662处理4小时剂量依赖性增加ACC磷酸化,其与脂肪酸
合成的抑制相关,IC50为3.2 μM。A-769662也会抑制小鼠肝细胞中脂肪酸合成,IC50为3.6 μM。[1] A-769662通过变构调节和抑制AMPK在Thr-172上的去磷酸化作用激活AMPK,类似于AMP的作用。[2] A-769662通过与AMPK无关的作用机制抑制蛋白酶体功能。 A-769662影响纯化26S蛋白酶体的体外活性,但是不影响纯化20S蛋白酶体的体外活性。A-769662对MEF细胞具有毒性作用。[3]一项最近的研究表明,A-769662抑制
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NGflfZY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cvWKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9zXCxcoTfZ4FzC:FSFXNRmw2OTJ3MESvK|5USU6JRWK8M4E, M3LZV2lvcGmkaYTpc44hd2ZiaIXtZY4hYUuJLUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3MDC9JFQ6Njl2MUKg{txONg ? NEHPSpI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cvWKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9zXCxcoTfZ4FzC:FSFXNRmw2OTJ3MESvK|5USU6JRWK8M4E,pCofilin / Cofilin / detyrosinated alpha tubulin / acetylated alpha tubulin ; PubMed: 26431377 Oncotarget A B. MCF-7 cells were treated with 100 400 M of A-769662 for 4-24 hours. Protein was harvested and Western blot analysis was done to determine levels of pAMPK at threonine 172, pCofilin at serine 3, detyrosinated alpha tubulin (glu-tub), and acetylated alpha tubulin (acetyl-tub). C D. BT-549 cells were treated with 100 400 M A-769662 for 4 24 hours. Protein was harvested and Western blot analysis was done to determine levels of markers listed above. p-AMPK / AMPK / p-S6K / S6K / p-ACC ; PubMed: 22456226 Neurosignals Representative immunoblots for p-AMPK, total AMPK, p-S6K, total S6K, p-ACC and actin under control conditions (C) and in response to 50 MA-769662 at various time points. A-769662对正常Sprague Dawley大鼠的短期治疗降低肝脏丙二酰CoA水平,和换气比值,VCO2/VO2,表明整体脂肪酸氧化比率增加。A-769662(30 mg/kg, b.i.d.)治疗ob/ob小鼠,减少PEPCK,G6Pase,和FAS的肝脏表达,降低40%血浆葡萄糖,减少体重增加,并显著降低血浆和肝脏甘油三酯水平。[1] 96-孔 AMPK 试验: AMPK活性通过监测SAMS
多肽底物(标准试验中为20 mM,附加试验中为100 mM)磷酸化根据前述方案(Anderson et al., 2004)测量。为测定 A-769662诱导的AMPK活化是否可逆,AMP或A-769662与大鼠肝脏AMPK在20倍标准试验浓度下预培养10分钟,然后稀释并测量AMPK活性。 Incubation Time: 24小时 Method: A-769662处理或未处理的MEF细胞的细胞活性如下进行测定:细胞通过胰蛋白酶化采集,并与0.5 mg/mL RNase 和50 μg/mL碘化丙啶在室温下黑暗中进行培育;细胞活性通过流式细胞术使用FACScanto流式细胞分析仪分析,激发激光波长为488 nm,碘化丙啶荧光检测波长为600 nm。为测定细胞周期各阶段的细胞比例,细胞通过胰蛋白酶化采集,通过离心收集,用PBS洗涤,并在80%乙醇中于-20°C固定过夜。随后,将这些固定的细胞离心以除去固定剂,并于黑暗中,室温下,在包含0.5 mg/mL RNase 和50 μg/mL碘化丙啶的PBS中培养20分钟。流式细胞术分析如上进行。G1,S,和G2期的细胞比例使用MODFIT程序测定。
细胞培养照片在指示时间使用结合具有20倍物镜的倒置
显微镜的相机拍摄。(Only for Reference) 从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献): 5% DMSO+40% PEG 300+5% Tween 80+50% ddH2O * 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。 第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方) 药物溶于 μL DMSO溶液(母液浓度 mg/mL,注:如该浓度超过该批次药物DMSO溶解度,请先联系Selleck); 体内配方配制方法:取 μL DMSO母液,加入 μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入 μL ddH2O,混匀澄清。 体内配方配制方法:取 μL DMSO母液,加入 μL Corn oil,混匀澄清。 注意:1. 首先保证母液是澄清的; 2. 一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。 本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为: 质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol) 摩尔浓度计算公式 *在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。 用本工具协助配置特定浓度的溶液,使用的计算公式为: 开始浓度 x 开始体积 = 最终浓度 x 最终体积 稀释公式 稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 ) 在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。. 连续稀释计算器方程 在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。 操作手册 Dorsomorphin (Compound C, BML-275)是一种有效的,可逆选择性 AMPK 抑制剂,无细胞试验中 Ki 为 109 nM,对几个结构相关的
激酶,包括ZAPK,SYK,PKCθ,PKA,和 JAK3没有显著的抑制作用。 也会抑制 I型BMP receptors ALK2,ALK3和ALK6 的活性。Dorsomorphin被应用于促进特定细胞分化和诱导癌细胞自噬。如需进行动物实验,建议选择可水溶产品 S7306 Dorsomorphin (Compound C) 2HCl。 CHIR-99021 (CT99021)是一种GSK-3α和GSK-3β抑制剂, IC50分别为10 nM and 6.7 nM。CHIR99201对CDKs没有交叉反应性,对GSK-3β的选择性是对CDKs选择性的350倍。CHIR-99021 可作为Wnt/β-catenin的激活剂并诱导自噬。 AICAR (Acadesine, NSC105823, AICA Riboside)是AMPK的激活剂,引起ZMP积累,模拟AMP对AMPK和AMPKK的刺激作用。AICAR (Acadesine)可诱导线粒体自噬。Phase 3。 Features:Acadesine is potentially first-in-class adenosine regulating agent (ARA)Phenformin (NSC-756501) HCl Phenformin HCl (NSC-756501, Phenethylbiguanide)是一种Phenformin的盐酸盐,是一种双胍类的抗糖尿病药。它能激活AMPK,提高其活性和磷酸化。 Dorsomorphin 2HCl (BML-275, Compound C) 是一种有效的,可逆的,选择性AMPK抑制剂,在无细胞试验中Ki为109 nM,对一些结构相关的激酶,包括ZAPK, SYK, PKCθ,PKA和JAK3没有显著的抑制作用。也会抑制I型BMP
受体。Dorsomorphin 可诱导癌细胞系的自噬。 Rapamycin (AY-22989, Rapamune, Sirolimus, NSC-2260804)是一种特定的 mTOR 抑制剂,在 HEK293细胞中,IC50 为 ~0.1 nM。 MK-2206 2HCl是一种高度选择性的Akt1/2/3抑制剂,在无细胞试验中IC50分别为8 nM/12 nM/65 nM;对250种其他蛋白激酶没有抑制活性。MK-2206 2HCl 在肿瘤细胞中可诱导自噬和凋亡。Phase 2。 Features:MK-2206是第一个进入
临床研究阶段的Akt
小分子变构抑制剂。 CHIR-99021 (CT99021) HCl是CHIR-99021的盐酸盐, 是GSK-3α/β抑制剂,IC50为10 nM/6.7 nM。其对GSK-3的选择性比对Cdc2和ERK2高500倍以上。CHIR-99021 是一种有效的药理性的 Wnt/beta-catenin 信号通路的激活剂。CHIR-99021 可显著地拯救光诱导的 autophagy,并可增强 GR,RORα和自噬相关蛋白等表达。 Tags: 购买A-769662 | A-769662供应商 | 采购A-769662 | A-769662价格 | A-769662生产 | 订购A-769662 | A-769662代理商